Sheep anti-Mouse IgG (H+L) Secondary Antibody [DyLight 800] (Pre-absorbed)

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Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG, H/L Chains Antibody [DyLight 800] [NBP1-72928]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
DyLight 800
Format
Pre-absorbed
Concentration
LYOPH

Sheep anti-Mouse IgG (H+L) Secondary Antibody [DyLight 800] (Pre-absorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 100 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional IgG (H+L) Products

IgG (H+L) NBP1-72928

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