Goat anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Janelia Fluor 549
Format
Pre-adsorbed

Goat anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed) Summary

Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72715JF549) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Allophycocyanin/Cy7 NBP1-72715APCCY7
DyLight 405 NBP1-72715V
DyLight 488 NBP1-72715G
DyLight 550 NBP1-72715R
DyLight 594 NBP1-72715DL594
DyLight 650 NBP1-72715C
DyLight 680 NBP1-72715FR
DyLight 755 NBP1-72715IR
Janelia Fluor 549 NBP1-72715JF549
Janelia Fluor 646 NBP1-72715JF646
PE/Atto594 NBP1-72715PEATT594
PE/Cy5.5 NBP1-72715PECY55
PE/Cy7 NBP1-72715PECY7

Additional IgG (H+L) Products

Array NBP1-72715JF549

Blogs on IgG (H+L)

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Recombinant Monoclonal Antibodies

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