Sheep anti-Rabbit IgG (H+L) Secondary Antibody [PE/Atto594]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Sheep
Conjugate
PE/Atto594

Sheep anti-Rabbit IgG (H+L) Secondary Antibody [PE/Atto594] Summary

Immunogen
This Sheep anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG whole molecule.
Specificity
Assay of Sheep anti-Rabbit IgG (H+L) Secondary Antibody by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep serum, Rabbit IgG and Rabbit serum.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

Alkaline Phosphatase NB120-6796
Allophycocyanin/Cy7 NBP1-72695APCCY7
Biotin NB120-6794
DyLight 405 NBP1-72695V
DyLight 488 NBP1-72695G
DyLight 550 NBP1-72695R
DyLight 594 NBP1-72695DL594
DyLight 650 NBP1-72695C
DyLight 680 NBP1-72695FR
DyLight 755 NBP1-72695IR
FITC NB120-6791
HRP NBP1-73253
Janelia Fluor 549 NBP1-72695JF549
Janelia Fluor 646 NBP1-72695JF646
PE/Atto594 NBP1-72695PEATT594
PE/Cy5.5 NBP1-72695PECY55
PE/Cy7 NBP1-72695PECY7
Rhodamine NB120-6792
Texas Red NB120-6793

Additional IgG (H+L) Products

Array NBP1-72695PEATT594

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