Goat F(ab')2 anti-Human IgG (H+L) Secondary Antibody [DyLight 649] (Pre-adsorbed)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 649
Format
Pre-adsorbed

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Goat F(ab')2 anti-Human IgG (H+L) Secondary Antibody [DyLight 649] (Pre-adsorbed) Summary

Immunogen
Human IgG-F(ab')2 Fragment
Specificity
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS containing 0.2% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Additional IgG (H+L) Products

IgG (H+L) NBP1-51902

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