Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) [NB7602] - Western Blot of Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine]. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine] at 1:1000 for 60 min at RT.Block: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): none.. Using the Rhodamine format of this antibody.

Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) [NB7602] - 0.62ngPrimary antibody: noneSecondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine] at 1:1000 for 60 min at RT.Block: Fluorescent blocking buffer for 60 min at RT.. Using the Rhodamine format of this antibody.

• Reactivity: Mu
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Cy5
• Format: Pre-adsorbed

Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) Summary

• Immunogen: Mouse IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Flow Cytometry 1:500-1:2500
  • Fluorophore-linked immunosorbent assay 1:10000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This product is designed for ICC/IF, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7602)(1)

Publication using NB7602

• Saribas AS, DeVoto J, Golla A Et al. Discovery and characterization of novel trans-spliced products of human polyoma JC virus late transcripts from PML patients J Cell Physiol 2017-10-19 [PMID: 29044559]
  
  Details:
  Citation using the Rhodamine version of this antibody.

Reviews for IgG (H+L) Antibody (NB7602) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Arun Satelli IHC-P Human 11/04/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human colonic tissue
• Species: Human

reviewed by: Arun Satelli Flow Human 10/16/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human colon cancer cell line HT29
• Species: Human

reviewed by: Arun Satelli ICC Human 10/16/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Human colon cancer cell line HT29
• Species: Human