Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG Antibody [Cy5] [NB7602]
Immunohistochemistry-Frozen: Goat anti-Mouse IgG Antibody [Cy5] [NB7602] - analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.
Flow Cytometry: Goat anti-Mouse IgG Antibody [Cy5] [NB7602]
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG Antibody [Cy5] [NB7602] - analysis of paraffin embedded human colonic tissue using Goat anti-Mouse IgG [Cy5] secondary antibody. Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications FLISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Cy5
Format
Pre-adsorbed

Order Details

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Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
Application Notes
This product is designed for ICC/IF, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB7602 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Background

This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species (human labelled with the fluorophore Cy5 here) in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB7602) (0)

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Reviews for IgG Antibody (NB7602) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB7602:
Filter by Applications
IHC-P
(1)
Flow
(1)
ICC
(1)
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Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IgG (H+L) NB7602
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reviewed by:
Arun Satelli
IHC-P Human 11/04/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman colonic tissue
SpeciesHuman
CommentsVery good secondary to detect in IHC paraffin section.

Blocking

Blocking DetailsFish Gelatin

Details

Detection NotesDetection using confocal microscope LSM510

Comments

CommentsVery good secondary to detect in IHC paraffin section.
Flow Cytometry IgG (H+L) NB7602
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reviewed by:
Arun Satelli
Flow Human 10/16/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman colon cancer cell line HT29
SpeciesHuman

Blocking

Blocking Details0.25% FBS in PBS

Details

Detection NotesDetection using flow cytometer
Fixation DetailsNo fixation, live cells stained with e-Cad first then secondary cy5
Wash Description5 min each 3 times in PBS
Immunocytochemistry IgG (H+L) NB7602
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reviewed by:
Arun Satelli
ICC Human 10/16/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman colon cancer cell line HT29
SpeciesHuman
CommentsAt 1:500, there is sustained view of E-Cadherin in the cells, however, if used at lower concentrations there is non-specific interactions that show up in all cells.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:500

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsCells were fixed in 4% PFA, PBS, Overnight 4 deg C
Wash Description5 min each 3 times in PBS

Comments

CommentsAt 1:500, there is sustained view of E-Cadherin in the cells, however, if used at lower concentrations there is non-specific interactions that show up in all cells.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG Antibody (NB7602) (0)

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Additional IgG (H+L) Products

IgG (H+L) NB7602

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Recent Reviews

4.3
5
2
4
0
3
1
2
0
1
0

Arun Satelli
11/04/2014
Application: IHC-P
Species: Human

Arun Satelli
10/16/2014
Application: Flow
Species: Human

Arun Satelli
10/16/2014
Application: ICC
Species: Human