Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP]

• Reactivity: Gt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP
• Concentration: 1.0 mg/ml

Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP] Summary

• Immunogen: This Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP] was developed against goat IgG-heavy and light chain.
• Specificity: By immunoelectrophoresis and ELISA this Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP] reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:5000
  • Western Blot 1:5000 - 1:50000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7352)(6)

Publications using NB7352

• Kato T, Yamada A, Sasa K et al. Nephronectin Expression is Inhibited by Inorganic Phosphate in Osteoblasts Calcif Tissue Int 2018-10-21 [PMID: 30341591]
• Kato T, Yamada A, Ikehata M et al. FGF-2 suppresses expression of nephronectin via JNK and PI3K pathways FEBS Open Bio 2018-05-11 [PMID: 29744297] (WB)
• Jin S, Zhu F, Wang Y et al. Deletion of Indian hedgehog gene causes dominant semi-lethal Creeper trait in chicken. Sci Rep. [PMID: 27439785] (WB)
  
  Details:
  Citation using the HRP form of this antibody.
• Li R, Ma Y, Zhai L et al. Enhanced immune response to foot-and-mouth disease vaccine by oral administration of ginseng stem-leaf saponins. Immunopharmacol Immunotoxicol. [PMID: 27216768]
  
  Details:
  Citation using the HRP form of this antibody.
• Nolasco LH, Turner NA, Bernardo A et al. Hemolytic uremic syndrome-associated Shiga toxins promote endothelial-cell secretion and impair ADAMTS13 cleavage of unusually large von Willebrand factor multimers. Blood. [PMID: 16131569]
  
  Details:
  Citation using the HRP form of this antibody.
• Yamagami S, Yokoo S, Mimura T et al. Effects of TGF-beta2 on immune response-related gene expression profiles in the human corneal endothelium. Invest Ophthalmol Vis Sci. [PMID: 14744893]
  
  Details:
  Citation using the HRP form of this antibody.

Review for IgG (H+L) Antibody (NB7352) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 03/26/2015

Summary

• Application: Western Blot
• Sample Tested: transfected U2OS cell lysate
• Species: Other