Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Product Discontinued

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Summary

• Immunogen: Rabbit IgG. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
• Localization: Secreted
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. This antibody may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:30000
• Application Notes: Use in IHC-Fr reported in scientific literature (PMID:33190582) This secondary antibody can be used for Western Blot, ELISA, and immunohisto/cytochemistry. Western Blots: Colorimetric: 1:1,000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:33190582)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate Buffered Saline (PBS), 0.2% BSA
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB730-H)(37)

Publications using NB730-H

• Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007-01-01 (WB)
• Abu-Elfotuh K, Al-Najjar AH, Mohammed AA et al. Fluoxetine ameliorates Alzheimer's disease progression and prevents the exacerbation of cardiovascular dysfunction of socially isolated depressed rats through activation of Nrf2/HO-1 and hindering TLR4/NLRP3 inflammasome signaling pathway International immunopharmacology 2022-01-15 [PMID: 35042170] (WB)
• Bahr HI, Ibrahiem AT, Gabr AM et al. Chemopreventive effect of alpha-hederin/carboplatin combination against experimental colon hyperplasia and impact on JNK signaling Sci Rep 2020-11-12 [PMID: 33190582] (IHC-Fr)
• Khumran AM, Yimer N, Rosnina Y et al. Butylated hydroxytoluene protects bull sperm surface protein-P25b in different extenders following cryopreservation Vet World 2020-06-18 [PMID: 32546907]
• Youssef MM, Abd El-Latif HA, El-Yamany MF, Georgy GS Aliskiren and captopril improve cognitive deficits in poorly controlled STZ-induced diabetic rats via amelioration of the hippocampal P-ERK, GSK3 beta, P-GSK3 beta pathway Toxicol. Appl. Pharmacol. 2020-05-01 [PMID: 32171570] (WB)
• Sun J, Niu L, Gao S et al. miR-509-5p Downregulation Is Associated With Male Infertility And Acts As A Suppressor In Testicular Germ Cell Tumor Cells Through Targeting MDM2 Onco Targets Ther 2019-12-02 [PMID: 31819532] (WB)
• Hajam IA, Kim J, Lee JH. Intranasally administered polyethylenimine adjuvanted influenza M2 ectodomain induces partial protection against H9N2 influenza A virus infection in chickens Vet. Immunol. Immunopathol. 2019-03-01 [PMID: 30885310] (WB)
• Li S, Wehrenberg B, Waldman BC et al. Mismatch tolerance during homologous recombination in mammalian cells DNA Repair (Amst.) 2018-08-01 [PMID: 30103093] (WB)
• Zhou Yan-Wu, Li Rong, Duan Chao-Jun et al. Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma. Molecular Medicine Reports 2017-01-01 [PMID: 28000881] (WB, WB)
• Ackermann Karin A, Bostock Hugh, Brander Lukas et al. Early changes of muscle membrane properties in porcine faecal peritonitis. Critical Care (London, England) 2014-01-01 [PMID: 25145497] (WB)
• Hulikova A, Swietach P. Rapid CO2 permeation across biological membranes: implications for CO2 venting from tissue. FASEB J. 2014-03-20 [PMID: 24652949] (WB)
• Muhammad SI, Maznah I, Mahmud RB et al. Estrogen receptor modulatory effects of germinated brown rice bioactives in the uterus of rats through the regulation of estrogen-induced genes. Drug Des Devel Ther 2013-12-02 [PMID: 24324328] (IHC-P)
• Chandler EM, Seo BR, Califano JP et al. Implanted adipose progenitor cells as physicochemical regulators of breast cancer Proc Natl Acad Sci U S A 2012-06-19 [PMID: 22665775] (IF/IHC, WB)
• Wang Y, Smith K, Waldman BC et al. Depletion of the bloom syndrome helicase stimulates homology-dependent repair at double-strand breaks in human chromosomes. DNA Repair (Amst). 2011-02-05 [PMID: 21300576] (WB)
• Gieseg SP, Amit Z, Yang YT et al. Oxidant Production, oxLDL Uptake, and CD36 Levels in Human Monocyte-Derived Macrophages Are Downregulated by the Macrophage-Generated Antioxidant 7,8-Dihydroneopterin. Antioxid Redox Signal. 2010-11-15 [PMID: 20408759] (WB)
• Werner DF, Swihart AR, Ferguson C et al. Alcohol-induced tolerance and physical dependence in mice with ethanol insensitive alpha1 GABA A receptors Alcohol Clin Exp Res. 2009-02-01 [PMID: 19032579] (WB)
• Wu C-C, Lu K-C, Chen J-S et al. HO-1 induction ameliorates experimental murine membranous nephropathy: anti-oxidative, anti-apoptotic immunomodulatory effects. Nephrol Dial Transplant;23(10):3082-3090. 2008-01-01 [PMID: 18477570] (IHC-P, WB)
• Homanics GE, Skvorak K, Ferguson C et al. Production and characterization of murine models of classic and intermediate maple syrup urine disease. BMC Med Genet. 2006-03-31 [PMID: 16579849] (WB)
• Skvorak, KJ. Investigation of gene and cellular therapies to cure maple syrup urine disease (MSUD) in a genetically engineered mouse model. PhD Thesis, University of Pittsburgh. 2008-01-01 (WB)
• Wu X, Liang L, Dong L et al. Effect of ARHI on lung cancer cell proliferation, apoptosis and invasion in vitro Mol Biol Rep 2012-12-18 [PMID: 23247805] (WB)
• Song J, Hao Y, Du Z et al. Identifying novel protein complexes in cancer cells using epitope-tagging of endogenous human genes and affinity-purification mass spectrometry J Proteome Res 2012-12-07 [PMID: 23106643] (WB)
• Song J, Saha S, Gokulrangan G et al. DNA and Chromatin Modification Networks Distinguish Stem Cell Pluripotent Ground States Mol Cell Proteomics 2012-07-22 [PMID: 22822199] (WB)
• Yassin AM, Hamid MIA, Farid OA, Amer H. Dromedary milk exosomes as mammary transcriptome nano-vehicle: Their isolation, vesicular and phospholipidomic characterizations. Journal of Advanced Research. 2015-11-02 (WB)
• Kamble NM, Hyoung KJ, Lee JH. Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus Vet. Res. 2017-08-07 [PMID: 28784184] (WB, Bacteria)
• Wang Y, Li S, Smith K et al. Intrachromosomal recombination between highly diverged DNA sequences is enabled in human cells deficient in Bloom helicase. DNA Repair (Amst.). 2016-05-01 [PMID: 27100209] (WB)
• Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia 2013-06-13 [PMID: 23756832] (WB)
• Rezaei M, Friedrich K, Wielockx B et al. Interplay between neural-cadherin and vascular endothelial-cadherin in breast cancer progression Breast Cancer Res 2012-12-06 [PMID: 23216791] (WB)
• Meng Q, Zheng M, Liu H et al. SASH1 regulates proliferation, apoptosis, and invasion of osteosarcoma cell Mol Cell Biochem 2012-10-29 [PMID: 23108792] (WB)
• Lee YD, Yoon JS, Yoon HH et al. Expression of Bis in the mouse gastrointestinal system Anat Cell Biol 2012-09-01 [PMID: 23094204] (IF/IHC)
• Gu T, Horova E, Mollsten A, Seman NA et al. IGF2BP2 and IGF2 genetic effects in diabetes and diabetic nephropathy J Diabetes Complications 2012-07-04 [PMID: 22770937] (WB)
• Gupta GK, Dhar K, Del Core MG et al. Suppressor of cytokine signaling-3 and intimal hyperplasia in porcine coronary arteries following coronary intervention. Exp Mol Pathol 2011-08-01 [PMID: 21540027] (WB)
• Aggarwal H, Aggarwal A, Hunter WJ 3rd et al. Expression of leukemia/lymphoma related factor (LRF/Pokemon) in human benign prostate hyperplasia and prostate cancer. Exp Mol Pathol 2011-04-01 [PMID: 21251909] (WB)
• Werner DF, Swihart A, Rau V et al. Inhaled anesthetic responses of recombinant receptors and knockin mice harboring {alpha}2(S270H/L277A) GABAA receptor subunits that are resistant to isoflurane. J Pharmacol Exp Ther. 2010-08-31 [PMID: 20807777]
• Aggarwal A, Hunter WJ 3rd, Aggarwal H et al. Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers Exp Mol Pathol 2010-10-01 [PMID: 20471975] (WB)
• Makinde TO, Agrawal DK. Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice. Am J Respir Cell Mol Biol. 2010-05-12 [PMID: 20463289] (ELISA)
• Ortiz-Capisano, MC et al. Calcium-dependent phosphodiesterase 1C inhibits renin release from isolated juxtaglomerular cells. Am J Physiol Regulatory Integrative Comp Physiol 297:R1469-R1476. 2009-01-01 [PMID: 19741056]
• Wen MJ, Lin YF, Chen JS. Newly developed hypertension as an early marker of recurrence of adrenocortical carcinoma with high renin expression. Int J Urol. 2008-06-01 [PMID: 18489644] (IHC-P)

Reviews for IgG Antibody (NB730-H) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Verified Customer WB Human 12/29/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23106643
• Species: Human

reviewed by: Wen Zhang WB Mouse 10/03/2014

Summary

• Application: Western Blot
• Sample Tested: mouse lung
• Species: Mouse

reviewed by: Verified Customer WB Other 10/12/2011

Summary

• Application: Western Blot
• Sample Tested: Rabbit
• Species: Other

FAQs for IgG Antibody (NB730-H). (Showing 1 - 1 of 1 FAQs).

1. How many test can be performed in 0.1 ml of PLA2R1 Antibody (NBP1-84449). Also kindly suggest the secondary Antibody for the same.
   • The number of tests using NBP1-84449 will depend upon the sample type (differential tissues expression) and the total volume of antibody used in each slide. This product is supplied in 100ul size and the concentration of the antibody is 0.4ug/ul and the working dilution we recommend for IHC is 1:500 to 1:1000, based on this information you should be able to estimate how many experiments can be done with one vial. As far as secondary antibody is concerned, any anti rabbit IgG secondary that is conjugated should technical work, the type of conjugation does not matter as much, HRP is most often used in labs while the biotin-conjugated antibody is used to amplify the signal which results in greater sensitivity than that achieved with an enzyme or fluorescence conjugated secondary antibody alone. Fluorescent labeled antibodies are commonly used for double or multiple staining methods. Here is the list of our secondary antibodies. I can recommend NB730-H as it has received very good reviews from our customers and it has been cited in 23 publications as well. I hope this answers your question. Please do not hesitate to contact us if you have any further questions.