Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC] (NB720-F)

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC] [NB720-F] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG ...read more
Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC] [NB720-F] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mosue IgG FITC conjugated secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
FITC
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Goat anti-Mouse IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Mouse IgG-heavy and light chain
Localization
Secreted
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against nonimmunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:50
  • Immunohistochemistry 1:50 - 1:50
  • Immunohistochemistry-Paraffin 1:50 - 1:500
Application Notes
Immunohistochemistry-Paraffin and Immunocytochemistry. Flow Cytometry was reported in scientific literature.
Reviewed Applications
Read 4 Reviews rated 4
using
NB720-F in the following applications:

Publications
Read Publications using
NB720-F in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS containing 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10-11 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB720-F)(5)

Reviews for IgG (H+L) Antibody (NB720-F) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 4 application: IHC-P, IHC-Fr, ICC, Flow.

Reviews using NB720-F:
Filter by Applications
IHC-P
(1)
IHC-Fr
(1)
ICC
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 11/11/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse tissue
SpeciesMouse
Immunohistochemistry-Frozen IgG (H+L) NB720-F
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reviewed by:
Anonymous
IHC-Fr Mouse 11/11/2014
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse tissue
SpeciesMouse
Immunocytochemistry IgG (H+L) NB720-F
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reviewed by:
Arun Satelli
ICC Human 10/09/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman primary Colon Cancer cell line
SpeciesHuman
CommentsI provided 2 different conditions, 1 at 1:50 and other at 1:100. Not big difference if you use a fluoroscence microscope, however if you use confocal, you can see the contrast of the image. However, very easily bleach-able so took pictures by looking at UV filter.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:50

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsCells were fixed in 4% PFA, PBS, Overnight 4 deg C
Wash Description5 min each 3 times in PBS

Comments

CommentsI provided 2 different conditions, 1 at 1:50 and other at 1:100. Not big difference if you use a fluoroscence microscope, however if you use confocal, you can see the contrast of the image. However, very easily bleach-able so took pictures by looking at UV filter.
Flow Cytometry IgG (H+L) NB720-F
Enlarge
reviewed by:
Arun Satelli
Flow Human 10/09/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman primary Colon Cancer cell line
SpeciesHuman
CommentsFor flow this antibody is very good. At dilutions of 1:100 works perfectly. At 1:50 I did observe some false negative on isotype, I am not sure what caused that. This is comparable to AF488.

Primary Anitbody

Dilution Ratio1:100

Details

Detection NotesDetection using flow cytometer
Fixation DetailsNo fixation, live cells stained with RYK first then secondary FITC
Permeabilization Detailsno permeabilization
Wash Description5 min each 3 times in PBS

Comments

CommentsFor flow this antibody is very good. At dilutions of 1:100 works perfectly. At 1:50 I did observe some false negative on isotype, I am not sure what caused that. This is comparable to AF488.

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB720-F) (0)

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Recent Reviews

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Anonymous
11/11/2014
Application: IHC-P
Species: Mouse

Anonymous
11/11/2014
Application: IHC-Fr
Species: Mouse

Arun Satelli
10/09/2014
Application: ICC
Species: Human