Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC]

Product Discontinued

Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC] Summary

• Immunogen: Mouse IgG-heavy and light chain
• Localization: Secreted
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against nonimmunoglobulin serum proteins. This may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:50
  • Immunohistochemistry 1:50 - 1:50
  • Immunohistochemistry-Paraffin 1:50 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS containing 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB720-F)(8)

Publications using NB720-F

• Bak DH, Lee E, Lee BC et al. Boehmite enhances hair follicle growth via stimulation of dermal papilla cells by upregulating beta-catenin signaling Exp. Dermatol. 2019-10-22 [PMID: 31638285]
• Kwon TR, Oh CT, Bak DH et al. Effects on skin of Stichopus japonicus viscera extracts detected with saponin including Holothurin A: Down-regulation of melanin synthesis and up-regulation of neocollagenesis mediated by ERK signaling pathway J Ethnopharmacol 2018-08-14 [PMID: 30102992] (ICC/IF, Mouse)
• Saribas AS, DeVoto J, Golla A Et al. Discovery and characterization of novel trans-spliced products of human polyoma JC virus late transcripts from PML patients J Cell Physiol 2017-10-19 [PMID: 29044559] (Mouse)
  
  Details:
  Citation using the Rhodamine version of this antibody.
• Kwon TR, Oh CT, Choi EJ et al. Human placental extract exerts hair growth-promoting effects through the GSK-3b signaling pathway in human dermal papilla cells. Int J Mol Med. [PMID: 26311045] (ICC/IF)
  
  Details:
  Citation using the FITC form of this antibody.
• Yoon J, Juhn KM, Ko JK et al. Effects of oxygen tension and IGF-I on HIF-1 alpha protein expression in mouse blastocysts J Assist Reprod Genet 2012-12-13 [PMID: 23232974] (IF/IHC, ICC/IF, Mouse)
• Wilkerson et al. Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules. Vet Immunol Immunopathol. [PMID: 15963817] (FLOW)
  
  Details:
  Citation using the FITC form of this antibody.
• Fukumoto et al. Identification and expression of a 50-kilodalton surface antigen of Babesia gibsoni and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. J Clin Microbiol. [PMID: 11427577]
  
  Details:
  Citation using the FITC form of this antibody.
• Flaminio MJ, Rush BR, Davis EG et al. Characterization of peripheral blood and pulmonary leukocyte function in healthy foals. Vet Immunol Immunopathol. [PMID: 10713340]
  
  Details:
  Citation using the FITC form of this antibody.

Reviews for IgG (H+L) Antibody (NB720-F) (4)

Average Rating: 4

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IHC-P Mouse 11/11/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse tissue
• Species: Mouse

reviewed by: Verified Customer IHC-Fr Mouse 11/11/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse tissue
• Species: Mouse

reviewed by: Arun Satelli ICC Human 10/09/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Human primary Colon Cancer cell line
• Species: Human

reviewed by: Arun Satelli Flow Human 10/09/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human primary Colon Cancer cell line
• Species: Human