Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]

Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]. Image from verified customer review.

• Reactivity: Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: 1.0 mg/ml

Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] Summary

• Immunogen: This Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] was developed against rat IgG-heavy and light chain.
• Specificity: By immunoelectrophoresis and ELISA this Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No was detected against non-immunoglobulin serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:5000
  • Immunohistochemistry 1:200-1:5000
  • Immunohistochemistry-Paraffin 1:200-1:5000
  • Western Blot 1:5000-1:50000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2% BSA
• Preservative: 0.05% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7115)(5)

Publications using NB7115

• Wong KM, King DA, Schwartz EK Et al. Retinoblastoma protein regulates carcinogen susceptibility at heterochromatic cancer driver loci Life Sci Alliance 2022-01-05 [PMID: 34983823] (WB)
  
  Details:
  Citation using the HRP version of this antibody.
• Z Digby, P Tourlomous, J Rooney, JP Boyle, B Bibo-Verdu, RJ Pickering, SJ Webster, TP Monie, LJ Hopkins, N Kayagaki, GS Salvesen, S Warming, L Weinert, CE Bryant Evolutionary loss of inflammasomes in the Carnivora and implications for the carriage of zoonotic infections Cell Reports, 2021-08-24;36(8):109614. 2021-08-24 [PMID: 34433041]
• O'Brien M, Baicu CF, Van Laer AO et al. Pressure-Overload Generates a Cardiac-Specific Profile of Inflammatory Mediators Am. J. Physiol. Heart Circ. Physiol. 2020-06-26 [PMID: 32589444] (IHC-P)
  
  Details:
  Citation using the HRP format of this antibody.
• Kim DY, Kim W, Lee KH et al. hnRNP Q regulates translation of p53 in normal and stress conditions. Cell Death Differ. 2013-02-01 [PMID: 22935615]
  
  Details:
  This citation used the HRP form of this antibody.
• Tsuji N, Suzuki K, Kasuga-Aoki H et al. Intranasal immunization with recombinant Ascaris suum 14-kilodalton antigen coupled with cholera toxin B subunit induces protective immunity to A suum infection in mice. Infect Immun. 2001-12-01 [PMID: 11705899]
  
  Details:
  This citation used the HRP form of this antibody.

Review for IgG (H+L) Antibody (NB7115) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/13/2015

Summary

• Application: Western Blot
• Sample Tested: SUSD3 Overexpression Lysate, NBL1-16623
• Species: Human
• Lot: P18