Rabbit anti-Mouse IgG (H+L) Secondary Antibody [HiLyte Fluor 647]


Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [HiLyte Fluor 647] [NB710-94917] - The tubulin in human glioblastoma cells was detected with mouse anti-tubulin primary antibody ...read more

Product Details

Reactivity MuSpecies Glossary
Applications ICC/IF
HiLyte Fluor 647
1 mg/ml

Order Details

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [HiLyte Fluor 647] Summary

Mouse IgG
Mouse IgG (H+L)
Immunogen affinity purified

Reactivity Notes

Reacts with mouse.

Packaging, Storage & Formulations

Store at 4C in the dark.
0.01 mM phosphate, 150 mM NaCl, pH 7.2, with bovine serum albumin.
2mM Sodium Azide
1 mg/ml
Immunogen affinity purified


HiLyte Plus(TM) 647 is a new fluorescent labeling dye with the structure designed for maximum brightness when conjugated to macromolecules. The fluorescence of HiLyte Plus(TM) 647 can be observed at excitation/emission wavelength of 649nm/669 nm and can be detected with filters designed for Cy5 dyes. HiLyte Plus(TM) 647-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. This IgG has minimal cross-reaction to human serum proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

FAQs for Mouse IgG Antibody (NB710-94917). (Showing 1 - 1 of 1 FAQ).

  1. What is the dilution factor and dilution buffer for this antibody?
    • The recommended concentration for immunofluorescent staining using NB710-94917 is 0.1-10 ug/ml. This antibody is supplied at 1mg/ml concentration. To block and to dilute the antibody we recommend using PBS-T containing 10% serum from the host species of the secondary antibody, but 1% BSA in PBS-T may also be used.

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Array NB710-94917

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