Chicken anti-Goat IgG (H+L) Secondary Antibody [FITC]

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
FITC
Concentration
1.0 mg/ml

Chicken anti-Goat IgG (H+L) Secondary Antibody [FITC] Summary

Immunogen
Chickens were immunized with purified, non-denatured goat IgG (containing both heavy and light chains).
Specificity
There is no detectable cross-reactivity with rabbit, human or mouse IgG.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Antibodies were tested for specificity using double- immunodiffusion in agarose and for non-specific binding using cryostat sections of unfixed embryonic chick tissues.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Purification Notes
Chickens were immunized with purified, non-denatured goat IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Goat IgGs were affinity-purified over a column conjugated with purified goat IgG (containing both heavy and light chains) and then conjugated with fluorescein isothiocyanate.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20C. Do not freeze these antibodies unless you want to store them for longer periods of time.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Other Available Formats

Allophycocyanin/Cy7 NB710-58357APCCY7
DyLight 405 NB710-58357V
DyLight 488 NB710-58357G
DyLight 550 NB710-58357R
DyLight 594 NB710-58357DL594
DyLight 650 NB710-58357C
DyLight 680 NB710-58357FR
DyLight 755 NB710-58357IR
FITC NB710-58353
HRP NB710-57835
Janelia Fluor 549 NB710-58357JF549
Janelia Fluor 646 NB710-58357JF646
PE/Atto594 NB710-58357PEATT594
PE/Cy5.5 NB710-58357PECY55
PE/Cy7 NB710-58357PECY7

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Array NB710-58353

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