Donkey F(ab')2 anti-Sheep IgG (H+L) Secondary Antibody [PE] (Pre-absorbed)

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Product Details

Summary
Reactivity ShSpecies Glossary
Applications Flow, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
PE
Format
Pre-absorbed

Order Details

Donkey F(ab')2 anti-Sheep IgG (H+L) Secondary Antibody [PE] (Pre-absorbed) Summary

Immunogen
Sheep IgG whole molecule
Specificity
Sheep IgG (H and L)
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:250
  • Immunocytochemistry/Immunofluorescence 1:100-1:250
Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required - stain 10^6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Reactivity Notes

Minimum Cross Reactivity to Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Can be used in immunomicroscopy and flow cytometry or analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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