Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800]

Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800] [NBP3-11666] - Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 800].

Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800] [NBP3-11666] - Lane 1: Rabbit IgG, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Rabbit IgG, Reduced. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 800] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: ~160 kDa for Rabbit IgG, Non-reduced.

Western Blot of Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800]. Lane 1: Rabbit IgG, Non-reduced. M: Opal Pre-stained Ladder

Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800] / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Rabbit TrueBlot(R): Anti-Rabbit IgG HRP

• Reactivity: Rb
• Applications: WB, DB, ICC/IF, IP
• Clone: eB182
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 800
• Concentration: LYOPH

Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800] Summary

• Description: This secondary antibody was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum.
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Rabbit IgG
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:2500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: This secondary antibody has been tested in dot blot, western blot, and immunoprecipitation and may also be used for detection in immunoassays that do not employ immunoprecipitation. This secondary antibody is provided as a lyophilized powder. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mLs/blot will yield enough reagent for 200 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit Pure-Blot will result in contaminating bands. For immunoprecipitation, Anti-rat IgG beads, or Anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. Bovine Serum Albumin, or blocking buffer for Fluorescent Western Blotting, at low concentrations, should be used as the blocking protein for the immunoblot. DO NOT USE BLOTTO or MILK. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. DyLight(TM) 800 and DyLight(TM) 680 antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. DyLight(TM) 800 conjugates are optimized for the Odyssey(R) Infrared Imaging System developed by LI-COR. DyLight(TM) 800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when DyLight(TM) 800 conjugates are used in conjunction with DyLight(TM) 680 conjugates. DyLight(TM) 800 and DyLight(TM) 680 conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Alternate Names for Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 800]

• IP Detection Reagent

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.