Rabbit IgG Fc Isotype Control [Texas Red]

SDS-Page: Rabbit IgG Fc Isotype Control [Texas Red] [NBP1-97232] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment Texas Red Conjugated. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.

Product Discontinued

Rabbit IgG Fc Isotype Control [Texas Red] Summary

• Description: This Rabbit IgG Fc immunoglobulin is useful as an isotype control for Rabbit IgG Fc antibody.
• Specificity: No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain.
• Isotype: IgG
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • SDS-Page 1:100 - 1:2000
• Application Notes: This isotype control is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Notes

This product was prepared from normal serum by delipidation, salt fractionation, and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F(c) and anti-Rabbit Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.