Human IgG Fc Isotype Control

SDS-Page: Human IgG Fc Isotype Control [NBP1-96973] - SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced Human IgG F(c) Fragment. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.

SDS-Page: Human IgG Fc Isotype Control [NBP1-96973] - SDS-PAGE of Human IgG Fc. Lane 1: Non-reduced Human IgG Fc Lane 2: Non-reduced Human IgG Whole Molecule Lane 3: Non-reduced Human IgG Fab Fragment Lane 4: Non-reduced Human IgG F(ab')2 Fragment. Middle Lane: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 5: Reduced Human IgG Fc Lane 6: Reduced Human IgG Whole Molecule Lane 7: Reduced Human IgG Fab Fragment Lane 8: Reduced Human IgG F(ab')2 Fragment. Load: 1ug per lane. Human IgG Whole Molecule, Human IgG Fab Fragment and Human IgG F(ab')2 Fragment ran as controls. Predicted/Observed size: Non-reduced at 50 kDa, reduced at 25 kDa/Non-reduced at 55-60 kDa, reduced at 30-33 kDa.

Dot Blot: Human IgG Fc Isotype Control [NBP1-96973] - Dot Blot: Human IgG Fc Isotype Control [Texas Red] [NBP1-97276] - Texas Red Conjugated Human IgG F(c). Load: 3-fold serial dilution starting at 200 ng. Image using the Texas Red form of this antibody.

Product Discontinued

Human IgG Fc Isotype Control Summary

• Description: Native Human IgG, Fc.

  Source: Serum

• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab)2 or anti-Papain.
• Isotype: IgG
• Host: Human
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000-1:50000
  • SDS-Page
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ion exchange chromatography

Notes

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.