Goat F(ab')2 anti-Human IgG Fc Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)

Product Discontinued

Goat F(ab')2 anti-Human IgG Fc Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed) Summary

• Immunogen: Purified Human IgG, Fc fragment
• Specificity: Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on human IgG. Cross Adsorbed: This antibody has been pre-adsorbed against bovine, horse, mouse and rabbit serum proteins, and mouse IgG2 to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, horse, mouse, rabbit, mouse1 IgG serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on human IgG.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Notes

Purity > 90% based on SDS-PAGE Small amounts of intact IgG may be present. . Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75015()
  • O75015()