Goat anti-Human IgG Fc fragment Secondary Antibody [HRP] (Pre-absorbed)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Format
Pre-absorbed
Concentration
LYOPH

Goat anti-Human IgG Fc fragment Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Purified Human IgG, Fc fragment
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on human IgG. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human IgA + IgM, bovine, horse, mouse, rabbit IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on human IgG.

Packaging, Storage & Formulations

Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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