Rabbit anti-Goat IgG Fc fragment Secondary Antibody [Janelia Fluor 549]

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Rabbit anti-Goat IgG Fc fragment Secondary Antibody [Janelia Fluor 549] Summary

Immunogen
Purified Goat IgG Fc fragment
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on goat IgG.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>95%, by SDS-PAGE

Notes

Purity > 95% based on SDS-PAGE. Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Fc fragment Antibody (NBP1-74853JF549) (0)

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Other Available Formats

Allophycocyanin/Cy7 NBP1-74853APCCY7
Biotin NBP1-74855
DyLight 350 NBP1-74853UV
DyLight 405 NBP1-74853V
DyLight 488 NBP1-74853G
DyLight 550 NBP1-74853R
DyLight 594 NBP1-74853DL594
DyLight 650 NBP1-74853C
DyLight 680 NBP1-74853FR
DyLight 755 NBP1-74853IR
HRP NBP1-74857
Janelia Fluor 549 NBP1-74853JF549
Janelia Fluor 646 NBP1-74853JF646
PE/Atto594 NBP1-74853PEATT594
PE/Cy5.5 NBP1-74853PECY55
PE/Cy7 NBP1-74853PECY7

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