Mouse anti-Human IgG Fc fragment Secondary Antibody (4A10)

Images

 
Western Blot: Mouse anti-Human IgG Fc fragment Secondary Antibody (4A10) [NBP1-51564] - Western blot analysis using human IgG (Fc specific) mouse mAb against different fusion proteins with human IgG(Fc specific) tag.
Immunocytochemistry/ Immunofluorescence: Mouse anti-Human IgG Fc fragment Secondary Antibody (4A10) [NBP1-51564] - Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Mouse anti-Human IgG Fc fragment Secondary Antibody (4A10) Summary

Immunogen
Purified fusion protein with human IgG(Fc Specific) tag.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Ascites

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Application Notes
This antibody is useful in Western Blot, ELISA, Immunofluorescence.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascitic fluid containing 0.03% sodium azide.
Preservative
0.03% Sodium Azide
Purity
Ascites

Background

The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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