Goat anti-Mouse IgG Fc Secondary Antibody [HRP]

• Reactivity: Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: LYOPH

Goat anti-Mouse IgG Fc Secondary Antibody [HRP] Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.
  
  Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
• Immunogen: Purified Mouse IgG Fc
• Specificity: Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
  • Immunohistochemistry-Paraffin
  • Immunomicroscopy 1:20-1:2000
  • Western Blot 1:200-1:5000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin mouse serum proteins, light chains on all mouse immunoglobulins, mouse IgG, F(ab)'2 fragment

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
• Preservative: 0.1% ProClin 150
• Concentration: LYOPH
• Purity: Affinity purified
• Reconstitution Instructions: Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IgG Fc fragment Antibody (NBP1-75179). (Showing 1 - 1 of 1 FAQ).

1. I am going to use mouse antibody IP from Hela cells, and then to perform western blot. The first antibody is mouse. My protein size is same as IgG light chain. I need anti-mouse heavy chain specific HRP to avoid the light chain. Could you tell me which reagent is better? Is that NBP1-75179 can avoid IgG light chain?
   • I have pulled up information on the antibody NBP1-75179 and believe this would be a great choice for you to use in your particular experiment. The lab has verified that it detects the heavy and not light chain and we would back this information that is indicated on the datasheet with our 100% guarantee.