Goat anti-Donkey IgG Fc Secondary Antibody [FITC]

Product Discontinued

Goat anti-Donkey IgG Fc Secondary Antibody [FITC] Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Donkey IgG Fc fragment
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG, Donkey IgG F(c) and Donkey Serum. No reaction occurred against Donkey IgG F(ab).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
• Application Notes: This product is designed for ICC/IF, FLISA and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Ion exchange chromatography

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 2.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.