Donkey anti-Goat IgG Fc Secondary Antibody

• Reactivity: Gt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Donkey
• Conjugate: Unconjugated
• Concentration: LYOPH

Donkey anti-Goat IgG Fc Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-donkey serum, Goat IgG, Goat IgG F(c) and Goat Serum
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Goat IgG F(c) fragment
• Specificity: No reaction was observed against Goat IgG F(ab).
• Isotype: Serum
• Clonality: Polyclonal
• Host: Donkey
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:2000 - 1:10000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Delipidation and Defibrination
• Reconstitution Instructions: Reconstitute with 2.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.