Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: Pre-adsorbed

Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed) Summary

• Description: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
• Immunogen: Human IgG Fc antiserum was solid phase adsorbed to ensure class specificity.
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:20000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000 - 1:20000
• Application Notes: Suggested starting dilutions are: ELISA: Coating: 1:100 - 1:500, Primary: 1:1000 - 1:30,000. Western Blots: Colorimetric: 1:1,000 - 1:10,000, Chemiluminescent: 1:1,000 - 1:30,000

Reactivity Notes

Minimum reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.