Goat anti-Mouse IgG Fc Secondary Antibody [HRP] (NB7561)

Images

 
Western Blot: Goat anti-Mouse IgG Fc Secondary Antibody [HRP] [NB7561] - analysis of Cadherin in Huh7 cell lysate. Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

Goat anti-Mouse IgG Fc Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG-Fc Fragment
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG. This may cross react with IgG from other species
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:50000
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
Application Notes
Chemiluminescent - use at a dilution of 1:1,000 - 1:30,000.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7561 in the following applications:

Publications
Read Publications using NB7561.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NB7561)(9)


Showing Publications 1 - 9 of 9.
Publications using NB7561 Applications Species
Uddowla S, Hollister J, Pacheco JM et al. A safe foot-and-mouth disease vaccine platform with two negative markers for differentiating infected from vaccinated animals. J Virol 2012 Nov [PMID: 22915802]
Munkhjargal T, Aboulaila M, Ueno A et al. Cloning and characterization of histone deacetylase from Babesia bovis. Vet Parasitol 2012 Dec [PMID: 22818786]
Lin SC, Lin YF, Chong P et al. Broader neutralizing antibodies against H5N1 viruses using prime-boost immunization of hyperglycosylated hemagglutinin DNA and virus-like particles. PLoS One 2012 [PMID: 22720032]
Sachais BS, Litvinov RI, Yarovoi SV et al. Dynamic antibody-binding properties in the pathogenesis of HIT. Blood 2012 Aug [PMID: 22577175]
Settem RP, El-Hassan AT, Honma K et al. Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. Infect Immun 2012 Jul [PMID: 22547549]
Sato K, Imai Y, Higashi N et al. Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a. Blood 2005 Jul [PMID: 15784728]
Tezuka H, Imai S, Muto R et al. Recombinant Dirofilaria immitis polyprotein that stimulates murine B cells to produce nonspecific polyclonal immunoglobulin E antibody. Infect Immun 2002 Mar [PMID: 11854206]
Zhou EM, Riva J, Clavijo A et al. Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus. Clin Diagn Lab Immunol 2001 May [PMID: 11329443]
Moratti E. Identification and Characterization of New Plasmatic Isoforms of the Tumor Suppressor Gene PTPRG and Development of New Specific Monoclonal Antibodies. Doctoral thesis. Universita' Degli Studi Di Verona. 43 p. 2011

Review for IgG Fc Antibody (NB7561) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB7561:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot IgG Fc NB7561
Enlarge
reviewed by:
Anonymous
WB Human 11/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuh7 whole cell lysate
SpeciesHuman

Blocking

Blocking Detailsblocked in 5% milk, 1 hr at RT

Primary Anitbody

Dilution Ratioprimary antibody against cadherin diluted 1/500 in 5% milk, 1 hr at RT

Secondary Antibody

Secondary Descriptionthis is the secondary

Details

Detection Notesfilm, exposed for 30 seconds

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Fc Antibody (NB7561) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Fc Products

Blogs on IgG Fc

There are no specific blogs for IgG Fc, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
11/12/2014
Application: WB
Species: Human

Customers Who Bought This Also Bought