Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed)

SDS-Page: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed) [NB120-6252] - SDS-PAGE results of Goat F(ab')2 anti-Rat IgG Fc Secondary antibody (Pre-adsorbed).Lane 1: reduced Goat F(ab')2 anti-Rat.Lane 2: Opal Prestained LadderLane 3: non-reduced Goat F(ab')2 anti-Rat.Load: 1.0ug.4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.

• Reactivity: Rt
• Applications: WB, ELISA, IHC, PAGE
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: Pre-adsorbed

Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed) Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Rat F(c) fragment in goat.
• Specificity: No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:1000 - 1:3000
  • SDS-Page
  • Western Blot 1:2000 - 1:5000
• Application Notes: This product has been tested by SDS=PAGE and is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.