Mouse anti-Human IgG Fc Secondary Antibody (EM-07)

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Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - IgG kappa light chain (1), IgG lambda light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
EM-07
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Catalog# & Conjugate Size Price

Mouse anti-Human IgG Fc Secondary Antibody (EM-07) Summary

Immunogen
Fusion protein of human IgG Fc fragment.
Specificity
The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Western Blot and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. The antibody EM-07 does not crossreact with IgM.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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