Rabbit IgG F(ab) Isotype Control

Images

 
Western Blot: Rabbit IgG, Fab Protein [NBP1-97016] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment ...read more
SDS-Page: Rabbit IgG, Fab Protein [NBP1-97016] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment (BULK ORDER). Lane 3: Reduced Rabbit IgG F(c) ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, IP, PAGE
Host
Rabbit
Conjugate
Unconjugated
Format
Purified
Concentration
2.0 mg/ml

Order Details

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Rabbit IgG F(ab) Isotype Control Summary

Description
Native Rabbit IgG, Fab.

Specificity
Rabbit IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(ab)2. No reaction was observed against anti-Rabbit IgG F(c) or anti-Papain.
Isotype
IgG
Host
Rabbit
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:100 - 1:2000
  • SDS-Page 1:100 - 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.0 mg/ml
Purity
Ion exchange chromatography

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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