Mouse IgG F(ab) Isotype Control [Biotin]

SDS-Page: Mouse IgG F(ab) Isotype Control [Biotin] [NBP1-97170] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment Biotin Conjugated. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane.

Product Discontinued

Mouse IgG F(ab) Isotype Control [Biotin] Summary

• Description: This Mouse IgG F(ab) immunoglobulin is useful as an isotype control for Mouse IgG F(ab) antibody.
• Specificity: No reaction was observed against anti-Mouse IgG F(c) or anti-Papain.
• Isotype: IgG
• Host: Mouse
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • SDS-Page 1:100 - 1:2000
• Application Notes: This isotype control can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Notes

This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.