Feline IgG F(ab) Isotype Control [HRP]

Product Discontinued

Feline IgG F(ab) Isotype Control [HRP] Summary

• Description: This Feline IgG F(ab) immunoglobulin is useful as an isotype control for Feline IgG F(ab) antibody.
• Specificity: No reaction was observed against anti-Cat IgG F(c) or anti-Papain.
• Isotype: IgG
• Host: Feline
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This isotype control can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Notes

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Cat IgG, anti-Cat IgG F(ab')2 and anti-Cat Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.