Chicken IgG F(ab) Isotype Control

SDS-Page: Chicken IgG F(ab) Isotype Control [NBP1-96773] - SDS-PAGE of Chicken IgG Fab Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.

• Reactivity: Ch
• Applications: WB, ELISA, PAGE
• Host: Chicken
• Conjugate: Unconjugated
• Format: Purified

Chicken IgG F(ab) Isotype Control Summary

• Description: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab')2 and anti-Chicken Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Specificity: No reaction was observed against anti-Chicken IgG F(c) or anti- Papain.
• Isotype: IgG
• Host: Chicken
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.