Goat anti-Human IgG F(ab) Secondary Antibody [HRP] (Azide Free)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-69435
    • Availability
      Product Discontinued

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Goat anti-Human IgG F(ab) Secondary Antibody [HRP] (Azide Free) Summary

Description
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Immunogen
Purified normal Fab of polyclonal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Human Fab of IgG.
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA 1:100-1:2000
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
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Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-69435 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG F(ab) Antibody (NBP2-69435) (0)

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Reviews for IgG F(ab) Antibody (NBP2-69435) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-69435:
Filter by Applications
ELISA
(1)
WB
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
ELISA IgG F(ab) NBP2-69435
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4
reviewed by:
Mona Agrawal
ELISA Human 11/23/2022
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Summary

ApplicationELISA
Sample TestedBlood plasma
SpeciesHuman
Lot16423
Western Blot IgG F(ab) NBP2-69435
Enlarge
5
reviewed by:
Mona Agrawal
WB Human 11/23/2022
View

Summary

ApplicationWestern Blot
Sample TestedNHBE cells
SpeciesHuman
Lot8283

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Recent Reviews

4.5
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1
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Mona Agrawal
11/23/2022
Application: ELISA
Species: Human

Mona Agrawal
11/23/2022
Application: WB
Species: Human