Goat IgG F(ab)2 Isotype Control

Immunocytochemistry/Immunofluorescence: Goat IgG F(ab)2 Isotype Control [NBP1-97023] - Positive Antibody Anti Goat IgG F(ab)2 Isotype Control . Image from verified customer review.

SDS-Page: Goat IgG F(ab)2 Isotype Control [NBP1-97023] - SDS-PAGE of Goat IgG F(ab')2 Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: Reduced Goat IgG F(c) Fragment. Lane 3: Reduced Goat IgG F(ab')2 Fragment. Lane 4: Reduced Goat IgM Whole Molecule. Load: 1 ug for IgG, F(c) and F(ab')2; 3 ug for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Product Discontinued

Goat IgG F(ab)2 Isotype Control Summary

• Description: Native Goat IgG F (ab)2 fragment.

  Source: Serum

• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(ab)2. No reaction was observed against anti-Goat IgG F(c) or anti- Pepsin.
• Isotype: IgG
• Host: Goat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • SDS-Page 1:100 - 1:2000
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Support By Application

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Review for IgG F(ab)2 Isotype Control (NBP1-97023) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 06/06/2018

Summary

• Lot: 33007