Rat IgG F(ab)2 Isotype Control

SDS-Page: Rat IgG F(ab)2 Isotype Control [NBP1-97015] - SDS-PAGE of Rat IgG F(ab')2 Fragment (0002). Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG F(ab')2 Fragment (0002). Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c) and F(ab')2; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

Product Discontinued

Rat IgG F(ab)2 Isotype Control Summary

• Description: Native Rat IgG, Fab2.

  Source: Serum

• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
• Isotype: IgG
• Host: Rat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page 1:100-1:2000
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Ion exchange chromatography

Background

Rat IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Rat IgG F(ab')2 molecules lack the Fc portion of Rat IgG and therefore receptors that bind Rat IgG Fc will not bind Rat IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Support By Application

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.