Porcine IgG F(ab)2 Isotype Control [Texas Red]

Product Discontinued

Porcine IgG F(ab)2 Isotype Control [Texas Red] Summary

• Description: This Porcine IgG F(ab) immunoglobulin is useful as an isotype control for Porcine IgG F(ab) antibody.
• Specificity: No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin.
• Isotype: IgG
• Host: Pig
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This isotype control is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)+D269G363E269:G11E269:G1108
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: For the 1 mg size, we recommend reconstitution with 1.0 ml deionized water. The 0.5 mg size is provided in liquid format (0.5 ml).

Notes

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab')2 and anti-Swine Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.