Golden Syrian Hamster IgG F(ab)2 Isotype Control

Product Discontinued

Golden Syrian Hamster IgG F(ab)2 Isotype Control Summary

• Description: Native Golden Syrian Hamster IgG, Fab2.

• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Golden Syrian Hamster IgG, anti-Golden Syrian Hamster IgG F(ab)2 and anti-Golden Syrian Hamster Serum. No reaction was observed against anti-Golden Syrian Hamster IgG F(c) or anti-Pepsin.
• Isotype: IgG
• Host: Golden Syrian Hamster
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

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Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.