Goat anti-Human IgG F(ab')2 Secondary Antibody [Biotin]

ELISA: Goat anti-Human IgG F(ab')2 Secondary Antibody [Biotin] - [NBP2-68497] - Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-Human Fab antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: 1 mg/ml

Goat anti-Human IgG F(ab')2 Secondary Antibody [Biotin] Summary

• Immunogen: Human IgG F(ab')2
• Specificity: Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity. The antibody demonstrates minimal cross-reactivity with bovine, horse and mouse IgG; other species have not been tested. No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:10000
  • Flow Cytometry 1:200 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:200 - 1:1000
  • Western Blot 1:1000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1.5% Bovine Serum Albumin
• Preservative: <0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P0DMV8(Human)