Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody [FITC] (Pre-adsorbed)

• Reactivity: Mu
• Applications: Flow, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: FITC
• Format: Pre-adsorbed
• Concentration: 1 mg/ml

Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody [FITC] (Pre-adsorbed) Summary

• Immunogen: Mouse IgG-F(ab')2 Fragment
• Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight fluorescein isothiocyanate (FITC). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry 1:50 - 1:500
  • Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500

Reactivity Notes

Mouse. Minimum reactivity to human and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2% BSA.
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.