Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody [HRP]

Product Discontinued

Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody [HRP] Summary

• Immunogen: Sheep IgG F(ab')2 fragment
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Frozen 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
  • Western Blot 1:1000 - 1:10000
• Application Notes: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:8000 to 1:32000 is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Ion exchange chromatography

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.