Rabbit anti-Canine IgG F(ab)2 Secondary Antibody [HRP]

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
LYOPH

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Rabbit anti-Canine IgG F(ab)2 Secondary Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Dog IgG F(ab')2 fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab)2 and Dog Serum. No reaction was observed against Dog IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Frozen 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
This product is designed for ICC/IF, FLISA and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 2.0 ml of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGHG