Rabbit anti-Canine IgG F(ab')2 Secondary Antibody

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Rabbit anti-Canine IgG F(ab')2 Secondary Antibody Summary

Immunogen
Dog IgG F(ab')2 fragment, rabbit anti Dog Fab2
Specificity
No reaction was observed against Dog IgG F(c).
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination

Applications/Dilutions

Dilutions
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:2000 - 1:10000
Application Notes
This secondary antibody are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 2.0 ml deionized water (or equivalent).

Notes

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Dog IgG, Dog IgG F(ab')2 and Dog Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Contact Information

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Calculators

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