Goat anti-Rabbit IgG F(ab)2 Secondary Antibody [HRP] (Pre-adsorbed)

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Western Blot: Goat F(ab')2 anti-Rabbit IgG Secondary Antibody [HRP] (Pre-absorbed) [NB120-6023] - Goat F(ab')2 anti-Rabbit IgG Secondary Antibody [HRP] [NB120-6023] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Format
Pre-adsorbed

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Goat anti-Rabbit IgG F(ab)2 Secondary Antibody [HRP] (Pre-adsorbed) Summary

Immunogen
Rabbit IgG F(ab')2 fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Frozen 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4000 to 1:20000 is suggested for this product.
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Reviewed Applications
Read 1 Review rated 4
using
NB120-6023 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Rabbit IgG F(ab)2 Secondary Antibody [HRP] (Pre-adsorbed)

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG Antibody (NB120-6023) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB120-6023:
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Images Ratings Applications Species Date Details
Western Blot IgG F(ab)2 NB120-6023
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reviewed by:
Anonymous
WB Human 10/09/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman cancer cell lysate
SpeciesHuman
Lot1803

Blocking

Blocking Details5% non-fat milk in TBS buffer, room temperature for 1 hour

Primary Anitbody

Dilution Ratio1:3000 in 5% non-fat milk in TBS buffer overnight at 4C (beta-Actin Antibody cell signaling #4967)

Secondary Antibody

Secondary Descriptionanti Rabbit IgG, Fab2 Antibody
Secondary Manufacturer Cat#NB120-6023
Secondary Concentration1:3000

Details

Detection Notes1 min exposure show band.

Comments

CommentsThis antibody reacts with rabbit IgG whole molecule very well. But it seems still has some reaction with Fc.

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FAQs for IgG Antibody (NB120-6023) (0)

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Bioinformatics Tool for IgG Antibody (NB120-6023)

Discover related pathways, diseases and genes to IgG Antibody (NB120-6023). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for IgG Antibody (NB120-6023)

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Blogs on IgG F(ab)2.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

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Anonymous
10/09/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGHG