Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c).
Immunogen affinity purified
Western Blot 1:1000 - 1:10000
ELISA 1:10000 - 1:50000
Immunohistochemistry 1:500 - 1:2500
Immunohistochemistry-Paraffin 1:500 - 1:2500
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:20,000 of the reconstitution concentration is suggested for this product.
Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post.
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