Mouse F(ab) anti-Human IgG Secondary Antibody (4A11) [PE]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clone
4A11
Clonality
Monoclonal
Host
Mouse
Conjugate
PE
Concentration
0.1 mg/ml

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Mouse F(ab) anti-Human IgG Secondary Antibody (4A11) [PE] Summary

Immunogen
Purified human IgG.
Specificity
The antibody 4A11 reacts with Fab fragment of human IgG.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Flow Cytometry 6 ug/ml
Application Notes
ELISA, Flow Cyt, ICC/IF, WB

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Phosphate buffered saline (PBS), approx. pH 7.4
Preservative
15mM Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Notes

Store in the dark. Avoid prolonged exposure to light.

Alternate Names for Mouse F(ab) anti-Human IgG Secondary Antibody (4A11) [PE]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Additional IgG Products

Research Areas for IgG Antibody (NBP1-44932)

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Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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