Sheep anti-Human IgD Fc Secondary Antibody

• Reactivity: Hu
• Applications: IHC, WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Sheep anti-Human IgD Fc Secondary Antibody Summary

• Description: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.
• Immunogen: Pools of purified homogenous IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to the Fc part of the IgD molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the immunogen, a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with human serum containing monoclonal IgD. No precipitation reaction is obtained with purified IgG, IgA, IgM, and IgG/Fab fragments.
• Clonality: Polyclonal
• Host: Sheep
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Immunoelectrophoresis
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Application Notes: Precipitation assays. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4C; prolonged storage at or below -20C. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  
  Precipitation assays

Reactivity Notes

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Whole Antiserum
• Reconstitution Instructions: Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.