| Reactivity | BvSpecies Glossary |
| Applications | ELISA, Func, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day. |
| Immunogen | Highly purified IgA/Secretory Component isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal bovine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal bovine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Whole Antiserum |
| Dilutions |
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| Application Notes | Precipitation assays. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitation assays. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Delipidated, heat inactivated lyophilized whole antiserum |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Whole Antiserum |
| Reconstitution Instructions | Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). |
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