Mouse IgA Kappa Isotype Control (MAK)

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SDS-Page: Mouse IgA Kappa Isotype Control (MAK) [NBP1-97030] - SDS PAGE Results of Mouse IgA Kappa isotype control (MAK). Lane 1: Mouse IgA Kappa isotype control (MAK) Reduced [1ug]. Lane 2: Opal Prestained Molecular ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, PAGE
Clone
MAK
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Purified

Order Details

Mouse IgA Kappa Isotype Control (MAK) Summary

Description
This Mouse IgA immunoglobulin is useful as an isotype control for Mouse IgA antibody.
Immunogen
Mouse IgA Kappa
Specificity
Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
Isotype
IgA
Clonality
Monoclonal
Host
Mouse
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
Application Notes
This isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Publications
Read Publication using NBP1-97030.

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative
0.1% Sodium Azide
Purity
Multi-step

Notes

This isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum.

Store vial at 4C prior to opening. Stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgA Kappa Isotype Control (NBP1-97030)(1)

We have publications tested in 1 application: Func.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Array NBP1-97030

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