Mouse IgA Kappa Isotype Control (MAK)

SDS-Page: Mouse IgA Kappa Isotype Control (MAK) [NBP1-97030] - SDS PAGE Results of Mouse IgA Kappa isotype control (MAK). Lane 1: Mouse IgA Kappa isotype control (MAK) Reduced [1ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgA Kappa isotype control (MAK) Non-Reduced [1ug]. 4-20% Gel, Coomassie Stained.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, PAGE
• Clone: MAK
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified
• Concentration: 1.0 mg/ml

Mouse IgA Kappa Isotype Control (MAK) Summary

• Description: This isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum.
  
  Store vial at 4C prior to opening. Stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Mouse IgA Kappa
• Specificity: Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
• Isotype: IgA
• Clonality: Monoclonal
• Host: Mouse
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgA Kappa Isotype Control (NBP1-97030)(1)

Publication using NBP1-97030

• Strieder-Barboza C, Baker NA, Flesher CG, et al. Advanced glycation end-products regulate extracellular matrix-adipocyte metabolic crosstalk in diabetes Sci Rep 2019-12-26 [PMID: 31875018] (Func)