Goat anti-Rat IgA Heavy Chain Secondary Antibody

Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None. Image using the Biotin form of this antibody.

Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): Rat IgA light chain. Image using the Texas Red form of this antibody.

• Reactivity: Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Goat anti-Rat IgA Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Rat IgA alpha heavy chain
• Specificity: No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:3000
  • Western Blot 1:1000-1:5000
• Application Notes: This product is suitable for Elisa, Western Blots and Immunohistochemistry. Antibody should be optimized by the end user for specific conditions of reactivity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IgA Heavy Chain Antibody (NBP2-60686). (Showing 1 - 1 of 1 FAQ).

1. One customer would like to know whether our product NBP1-73672 could be used directly as primary antibody to detect rat IgA of IHC Frozen section. As we know this antibody is NBP2-60686 conjugated with Rhodamine, NBP2-60686 is available for IHC-Fr as the datasheet says. The other question is Pre-adsorbed mean as NBP1-73672 is Goat anti-Rat IgA Secondary Antibody [Rhodamine] (Pre-adsorbed).
   • NBP1-73672 is indeed NBP2-60686 conjugated with Rhodamine and both can be used in IHC as secondaries. No testing has been done using this antibody as primary hence we cannot give our guarantee that it will work. My main concern here would be extra background. Pre-adsorbed here means that "Anti-Rat IgA was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum.  No reaction was observed against Rat IgM or Rat IgG.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes".