Goat anti-Ferret IgA Heavy Chain Secondary Antibody [HRP]

Product Discontinued

Goat anti-Ferret IgA Heavy Chain Secondary Antibody [HRP] Summary

• Immunogen: Ferret IgA alpha heavy chain
• Specificity: Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Frozen 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
  • Western Blot 1:1000 - 1:5000
• Application Notes: This product has been assayed against 1.0 ug of Ferret IgA in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE as a substrate for 30 minutes at room temperature. A working dilution of 1:2000 to 1:8000 of the reconstitution concentration is suggested for Anti-Ferret IgA (alpha chain) Antibody.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Thimerosal
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml of deionized water.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IgA Antibody (NBP1-73728)(1)

Publication using NBP1-73728

• Nachbagauer R, Liu WC, Choi A et al. A universal influenza virus vaccine candidate confers protection against pandemic H1N1 infection in preclinical ferret studies NPJ Vaccines 2017-12-22 [PMID: 29263881] (ELISA, Ferret)