Rabbit anti-Mouse IgA Heavy Chain Secondary Antibody

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Rabbit anti-Mouse IgA Heavy Chain Secondary Antibody Summary

• Immunogen: Mouse IgA
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with mouse IgA. This may cross react with IgA from other species.
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Coating - 1:100-1:500 Primary - 1:1000-1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000-1:30000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgA Antibody (NB7506)(11)

Publications using NB7506

• Steimle A, Neumann M, Grant E et al. Gut microbiome-based prediction of autoimmune neuroinflammation bioRxiv 2023-04-17
• Steimle, A;Neumann, M;Grant, ET;Willieme, S;De Sciscio, A;Parrish, A;Ollert, M;Miyauchi, E;Soga, T;Fukuda, S;Ohno, H;Desai, MS; Gut microbial factors predict disease severity in a mouse model of multiple sclerosis Nature microbiology 2024-07-15 [PMID: 39009690]
• Gabriel Vasconcelos Pereira, Marie Boudaud, Mathis Wolter, Celeste Alexander, Alessandro De Sciscio, Erica T Grant, Bruno Caetano Trindade, Nicholas A Pudlo, Shaleni Singh, Austin Campbell, Mengrou Shan, Li Zhang, Qinnan Yang, Stéphanie Willieme, Kwi Kim, Trisha Denike-Duval, Jaime Fuentes, André Bleich, Thomas M Schmidt, Lucy Kennedy, Costas A Lyssiotis, Grace Y Chen, Kathryn A Eaton, Mahesh S Desai, Eric C Martens Opposing diet, microbiome, and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host. Cell host & microbe 2024-04-15 [PMID: 38513656]
• Pereira GV, Boudaud M, Wolter M et al. Unravelling specific diet and gut microbial contributions to inflammatory bowel disease Research Square 2023-05-26 [PMID: 36993463]
• McGinley LM, Chen KS, Mason SN et al. Monoclonal antibody-mediated immunosuppression enables long-term survival of transplanted human neural stem cells in mouse brain Clinical and Translational Medicine 2022-09-13 [PMID: 36101963]
• Lei Z, Liu W, Nie Y et al. EpCAM Is Essential to Maintaining the Immune Homeostasis of Intestines via Keeping the Expression of pIgR in the Intestinal Epithelium of Mice Frontiers in Immunology 2022-04-13 [PMID: 35493520]
• Kaaij MH, Rip J, Jeucken KCM et al. Overexpression of Transmembrane TNF Drives Development of Ectopic Lymphoid Structures in the Bone Marrow and B Cell Lineage Alterations in Experimental Spondyloarthritis Journal of immunology (Baltimore, Md. : 1950) 2021-09-24 [PMID: 34561228]
• Lei Z, Yang L, Lei Y et al. High Dose Lithium Chloride Causes Colitis through Activating F4/80 Positive Macrophages and Inhibiting Expression of Pigr and Claudin-15 in the Colon of Mice Toxicology 2021-04-23 [PMID: 33901603]
• Magri G, Comerma L, Pybus M et al. Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals Immunity 2017-07-16 [PMID: 28709802]
• Bice BD, Stephens MR, Georges SJ et al. Environmental Enrichment Induces Pericyte and IgA-Dependent Wound Repair and Lifespan Extension in a Colon Tumor Model Cell Rep 2017-04-27 [PMID: 28445727] (IF/IHC)
• Hossain MJ, Mori I, Dong L et al. Egg-adapted replication-restricted virus protects mice against lethal influenza. Vaccine 2000-07-01 [PMID: 10856787]