Rabbit anti-Human IgA Fc Secondary Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IEP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Rabbit anti-Human IgA Fc Secondary Antibody Summary

Immunogen
Highly purified normal IgA isolated from pooled human serum and a purified IgA2- . Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum and milk, a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments.
Clonality
Polyclonal
Host
Rabbit
Purity
Whole Antiserum

Applications/Dilutions

Dilutions
  • Immunoelectrophoresis
Application Notes
Precipitation assays

Reactivity Notes

The antiserum does not cross-react with any other component of the Human immunoglobulin system as tested in gel-immunodiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Delipidated, heat inactivated, lyophilized, stable whole antiserum
Preservative
No Preservative
Concentration
LYOPH
Purity
Whole Antiserum
Reconstitution Instructions
Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Notes

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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